Infection with the hepatitis C virus (HCV) remains a considerable public health concern in the Middle East andNorth Africa (MENA). The objectives of this study were to analyze the HCV genotype (GT) and sub-genotype(SGT) distribution in the MENA region and to assess the temporal change in the number of sequences withinthe MENA region. All HCV molecular sequences collected in the MENA region had been retrieved from GenBankas of 1 August 2022. The number of HCV sequences retrieved was 6740 representing sequences from a total of 17MENA countries with a majority from Iran (n = 1969, 29.2%), Egypt (n = 1591, 23.6%), Tunisia (n = 1305,19.4%) and Saudi Arabia (n = 1085, 16.1%). The determination of GT/SGT was based on the NCBI genotypingand Blast tool. Genotype 1 (GT1) dominated infections in the MENA (n = 2777, 41.2%), followed by GT4 (n =2566, 39.0%). Additionally, SGT4a (1515/6393, 23.7%) was the most common SGT in the MENA, and SGT4awas dominant in Egypt and Saudi Arabia, followed by SGT1b