Summary: A study in Lebanon assessed healthcare workers' (HCWs) knowledge and attitude towards MPOX and their conspiratorial attitudes towards emerging virus infections (EVIs). Out of 646 participants, one-third demonstrated good knowledge about MPOX, while a similar proportion had a satisfactory attitude. 25.4% endorsed conspiracy beliefs towards EVIs. Factors like postgraduate education and older age were linked to better MPOX knowledge and attitude. Physicians showed higher MPOX knowledge levels. Male sex, being a physician, and postgraduate education were associated with lower endorsement of conspiracy beliefs. Improved MPOX knowledge correlated with a positive attitude towards the disease. The study suggests addressing educational gaps to enhance HCWs' response to MPOX in Lebanon. Although conspiracy beliefs were less prevalent among HCWs, awareness of their impact on healthcare-seeking behavior is crucial.