Background Antibiotic resistance (AR) is a major global concern. Indiscriminate use of antibiotics may contribute to treatment failure and AR. Data about community pharmacists’ (CPs) knowledge of and behaviour toward antibiotic use and AR are limited. Objective Our objective was to evaluate the knowledge of and attitudes towards antibiotic use and AR in CPs in Jordan. Methods A large cross-sectional face-to-face survey of a random sample of 500 CPs in Jordan was conducted over a 2-month period. A representative sample was collected using the proportionate random sampling technique, which enabled us to geographically categorize the study population. Reliability and validity measures were taken to ensure a comprehensive and appropriate study tool. We used the Statistical Package for Social Science (SPSS®) version 24 to conduct descriptive analysis and logistic regression. Results The majority (86.6%) of respondents thought it was legal to dispense antibiotics without a prescription in Jo