Aim: To isolate and identify Listeria monocytogenes from frozen and shock frozen raw
dressed broiler chicken in Khartoum State, Sudan.
Place and Duration of Study: Department of Pathology, Microbiology and Parasitology,
Sudan University of Science and Technology, Khartoum-North, Sudan, between July 2011
and June 2012.
Methodology: Eight hundred samples were used in this the study. Five hundred frozen
(-18º) raw dressed broiler chickens samples were collected from five station chicken
abattoirs in Khartoum State. Three hundred samples were collected as fresh, -18ºC and
shock frozen (-40ºC) raw dressed broiler chickens. Detection and isolation of L.
monocytogenes was carried out using the conventional International Organization for
Standardization method.
Results: Out of the 500 samples, 195(39%) were found to be contaminated with Iisteria
spp.; L. monocytogenes 64(12.8%), Listeria ivanovii 97(19.4%), Listeria grayi 20(4%),
Listeria seeligeri 5(1%), Listeria welshimeri 9(1.8%). Out of the 300 samples, 111(37%) were found to be contaminated with Iisteria spp.; L. monocytogenes 39(13%), Listeria
ivanovii 54(18%), Listeria grayi 11(3.6%), Listeria seeligeri 3(1%) and Listeria welshimeri
4(1.3%).
Conclusion: The results presented in this study indicated that L. monocytogenes was found in frozen (-18ºC) raw dressed broiler chicken and shock frozen (-40ºC) raw dressed
broiler chickens.
